With the support of the Lower Mekong Initiative (LMI) Embassy Regional Grants Program, SEAOHUN in #collaboration with THOHUN organized the Training of Trainers (TOT) on OneHealth (OH) Approach & OH Core Competencies (OHCCs) in Vientiane, Lao PDR on 27-29 May 2019. Twenty-eight participants from various disciplines from the University of Health Sciences, National University of Laos and Ministry of Health joined the three-day training of trainers. The participants were equipped with practical tools and activities they may use to teach OHCCs and a step-by-step on how to develop an OHCC-based course starting with curriculum mapping to course development. At the end of the training, all the participants were encouraged to apply what they learned in the courses they teach. The training ended positively with seven faculty members volunteering to train their peers in the future or provide a cascade training and become master trainers with coaching from regional experts.